بررسی اثر عصاره مغز جنین بر نرون های هسته عصب هیپوگلوس ضایعه دیده در رت (Rat)

Background and purpose: Lesion on neural fiber is a phenomenon which can lead to cell death of the relevant functional neurons through a retrograde degeneration. Ïn order to show the probable effect of existing trophic factors present in immature brain (fetus) in maintaining and existence of mature motor neurons, this study was conducted experimentally on animals. Materials and Methods: Ïn this study we transected the hypoglossal nerve in 12 males wistar rat of 2 months old. The rats were divided into two, Çontrol and experimental groups. They were injected with 0.1 ml of fetal brain extract daily for 14 days. Ând in the same way the control group received normal saline. Âfter two weeks all the rats were anesthetized and perfused with formalin (10%). Âfter fixation, the brain stem of all rats were dissected, processed and serially sectioned (with 5 micron thickness), stained and then pericaryon of neurons of hypoglossal neucleus were counted and compared the unoperated and operated side in all the cases. Results: The result of this research showed that there was considerable reduction of the number of the neurons in the nuclei of the hypoglossal which had lesions in the control group as compare to the experimental group. Çonclusion: This result indicates that in spite of disconnection of axonal neurons with the target organ in the experimental group probably via proximal end of transected nerves, and through axonal transport, fetal brain drived trophic factors, transport to pericaryons of neurons and therefore prevent them from neuronal death in experimental group.